Article
Characterization of the trypsin-III from Monterey sardine (Sardinops caeruleus): Insights on the cold-adaptation from the A236N mutant.
International journal of biological macromolecules - 1 Dec 2020
Carretas-Valdez Manuel I, Moreno-Cordova Elena N, Ibarra-Hernandez Brisa G, Cinco-Moroyoqui Francisco J, Castillo-Yañez Francisco J, Casas-Flores Sergio, Osuna-Amarillas Pablo S, Islas-Osuna Maria A, Arvizu-Flores Aldo A
Abstract excerpt
Trypsins (E.C. 3.4.21.4) are digestive enzymes that catalyze the hydrolysis of peptide bonds containing arginine and lysine residues. Some trypsins from fish species are active at temperatures just above freezing, and for that are called cold-adapted enzymes, having many biotechnological applications. In this work, we characterized a recombinant trypsin-III from Monterey sardine (Sardinops caeruleus) and studied...
Topics
- Animals
- Cold Temperature
- Enzyme Activation
- Enzyme Stability
- Fish Proteins
- Fishes
- Hydrogen Bonding
- Models, Molecular
- Molecular Docking Simulation
- Mutation
- Protein Structure, Secondary
- Trypsin