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Article

Role of disulphide bonds in a thermophilic serine protease aqualysin I from Thermus aquaticus YT-1.

Journal of biochemistry - 1 May 2008

Sakaguchi Masayoshi, Takezawa Makoto, Nakazawa Rie, Nozawa Kazutaka, Kusakawa Taro, Nagasawa Takeshi, Sugahara Yasusato, Kawakita Masao

Abstract excerpt

A thermophilic serine protease, Aqualysin I, from Thermus aquaticus YT-1 has two disulphide bonds, which are also found in a psychrophilic serine protease from Vibrio sp. PA-44 and a proteinase K-like enzyme from Serratia sp. at corresponding positions. To understand the significance of these disulphide bonds in aqualysin I, we prepared mutants C99S, C194S and C99S/C194S (WSS), in which Cys69-Cys99, Cys163-Cys194...

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PMID: 18216068
DOI: 10.1093/jb/mvn007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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