Article
The effect of deleting a putative salt bridge on the properties of the thermostable subtilisin-like proteinase, aqualysin I.
Protein and peptide letters - 1 Jun 2011
Arnórsdóttir Jóhanna, Magnúsdóttir Manuela, Friđjónsson Olafur H, Kristjánsson Magnús M
Abstract excerpt
Aqualysin I, is a subtilisin-like serine proteinase, from the thermophilic bacterium Thermus aquaticus. It is predicted that the enzyme contains a salt bridge, D17-R259, connecting the N- and C-terminal regions of the enzyme. Previously we reported on the stabilizing effect of the incorporation of a salt bridge at a corresponding site in VPR, a related cold adapted enzyme from a marine Vibrio sp. Here we describe...
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