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Article

Stepwise adaptations to low temperature as revealed by multiple mutants of psychrophilic α-amylase from Antarctic Bacterium.

The Journal of biological chemistry - 4 Nov 2011

Cipolla Alexandre, D'Amico Salvino, Barumandzadeh Roya, Matagne André, Feller Georges

Abstract excerpt

The mutants Mut5 and Mut5CC from a psychrophilic α-amylase bear representative stabilizing interactions found in the heat-stable porcine pancreatic α-amylase but lacking in the cold-active enzyme from an Antarctic bacterium. From an evolutionary perspective, these mutants can be regarded as structural intermediates between the psychrophilic and the mesophilic enzymes. We found that these engineered interactions...

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Identifiers

PMID: 21900238
DOI: 10.1074/jbc.M111.274423
PMCID: PMC3207396

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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