Article
Enhancement of angucycline production by combined UV mutagenesis and ribosome engineering and fermentation optimization in Streptomyces dengpaensis XZHG99T.
Preparative biochemistry & biotechnology - 1 Jan 2021
Li Yumei, Li Jiyu, Ye Zhengmao, Lu Lingchao
Abstract excerpt
Strain improvement of Streptomyces dengpaensis XZHG99T was performed by combined UV mutagenesis and ribosome engineering, as well as fermentation optimization for enhanced angucycline production (rabelomycin and saquayamycin B1). First, four streptomycin-resistant mutants were obtained after screening of UV mutagenesis and ribosome engineering. Then a rpsL mutant (HTT7) with higher productivity of rabelomycin and...
Topics
- Anthraquinones
- Anti-Bacterial Agents
- Chromatography, High Pressure Liquid
- Drug Resistance, Bacterial
- Fermentation
- Hydrogen-Ion Concentration
- Mutagenesis
- Mutation
- Phenotype
- Ribosomes
- Streptomyces
- Ultraviolet Rays