Article
Strain improvement of Streptomyces roseosporus for daptomycin production by rational screening of He-Ne laser and NTG induced mutants and kinetic modeling.
Applied biochemistry and biotechnology - 1 Mar 2011
Yu Guanghai, Jia Xiaoqiang, Wen Jianping, Lu Wenyu, Wang Guoying, Caiyin Qinggele, Chen Yunlin
Abstract excerpt
To improve the yield of daptomycin by Streptomyces roseosporus, a method of rational screening of He-Ne Laser and N-methyl-N-nitro-N-nitrosoguanidine (NTG)-induced mutants was employed in this work. Under the optimal mutagenesis conditions (NTG of 0.3 mg/L, 40 min; irradiation at 15 mW, 15 min), two steps of combined mutations were conducted. Screening of mutants was done according to the individual resistance to...
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