Article
Genome shuffling based on different types of ribosome engineering mutants for enhanced production of 10-membered enediyne tiancimycin-A.
Applied microbiology and biotechnology - 1 May 2020
Liu Huiming, Jiang Chengzhou, Lin Jing, Zhuang Zhoukang, Kong Wenping, Liu Ling, Huang Yong, Duan Yanwen, Zhu Xiangcheng
Abstract excerpt
Tiancimycin-A (TNM-A) is an anthraquinone-fused ten-membered enediyne produced by Streptomyces sp. CB03234, which is very promising for the development of anticancer antibody-drug conjugates (ADCs). To improve the titer of TNM-A, we have generated high-producing mutants CB03234-S and CB03234-R through ribosome engineering, but still not sufficient for pilot production of TNM-A. As the follow-up work,...
Topics
- Biosynthetic Pathways
- DNA Shuffling
- Enediynes
- Fermentation
- Genetic Engineering
- Genome, Bacterial
- Mutation
- Ribosomes
- Streptomyces