Article
Mapping the Effects of Genetic Variation on Chromatin State and Gene Expression Reveals Loci That Control Ground State Pluripotency.
Cell stem cell - 3 Sept 2020
Skelly Daniel A, Czechanski Anne, Byers Candice, Aydin Selcan, Spruce Catrina, Olivier Chris, Choi Kwangbom, Gatti Daniel M, Raghupathy Narayanan, Keele Gregory R, Stanton Alexander, Vincent Matthew, Dion Stephanie, Greenstein Ian, Pankratz Matthew, Porter Devin K, Martin Whitney, O'Connor Callan, Qin Wenning, Harrill Alison H, Choi Ted, Churchill Gary A, Munger Steven C, Baker Christopher L, Reinholdt Laura G
Abstract excerpt
Mouse embryonic stem cells (mESCs) cultured in the presence of LIF occupy a ground state with highly active pluripotency-associated transcriptional and epigenetic circuitry. However, ground state pluripotency in some inbred strain backgrounds is unstable in the absence of ERK1/2 and GSK3 inhibition. Using an unbiased genetic approach, we dissect the basis of this divergent response to extracellular cues by...
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