Article
Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies.
Nature biomedical engineering - 1 Sept 2020
Tanaka Nobuyuki, Kanatani Shigeaki, Kaczynska Dagmara, Fukumoto Keishiro, Louhivuori Lauri, Mizutani Tomohiro, Kopper Oded, Kronqvist Pauliina, Robertson Stephanie, Lindh Claes, Kis Lorand, Pronk Robin, Niwa Naoya, Matsumoto Kazuhiro, Oya Mototsugu, Miyakawa Ayako, Falk Anna, Hartman Johan, Sahlgren Cecilia, Clevers Hans, Uhlén Per
Abstract excerpt
Microscopy analysis of tumour samples is commonly performed on fixed, thinly sectioned and protein-labelled tissues. However, these examinations do not reveal the intricate three-dimensional structures of tumours, nor enable the detection of aberrant transcripts. Here, we report a method, which we name DIIFCO (for diagnosing in situ immunofluorescence-labelled cleared oncosamples), for the multimodal volumetric...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.