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Directed Evolution of Ornithine Cyclodeaminase Using an EvolvR-Based Growth-Coupling Strategy for Efficient Biosynthesis of l-Proline.

ACS synthetic biology - 17 Jul 2020

Long Mengfei, Xu Meijuan, Qiao Zhina, Ma Zhenfeng, Osire Tolbert, Yang Taowei, Zhang Xian, Shao Minglong, Rao Zhiming

Abstract excerpt

l-Proline takes a significant role in the pharmaceutical and chemical industries as well as graziery. Typical biosynthesis of l-proline is from l-glutamate, involving three enzyme reactions as well as a spontaneous cyclization. Alternatively, l-proline can be also synthesized in l-ornithine and/or l-arginine producing strains by an ornithine aminotransferase (OCD). In this study, a strategy of directed evolution...

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PMID: 32551572
DOI: 10.1021/acssynbio.0c00198

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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