Article
Quantification of ongoing APOBEC3A activity in tumor cells by monitoring RNA editing at hotspots.
Nature communications - 12 Jun 2020
Jalili Pégah, Bowen Danae, Langenbucher Adam, Park Shinho, Aguirre Kevin, Corcoran Ryan B, Fleischman Angela G, Lawrence Michael S, Zou Lee, Buisson Rémi
Abstract excerpt
APOBEC3A is a cytidine deaminase driving mutagenesis, DNA replication stress and DNA damage in cancer cells. While the APOBEC3A-induced vulnerability of cancers offers an opportunity for therapy, APOBEC3A protein and mRNA are difficult to quantify in tumors due to their low abundance. Here, we describe a quantitative and sensitive assay to measure the ongoing activity of APOBEC3A in tumors. Using hotspot RNA...
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