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Article

Tutorial: design and execution of CRISPR in vivo screens.

Nature protocols - 1 Sept 2022

Braun Christian J, Adames Andrés Carbonell, Saur Dieter, Rad Roland

Abstract excerpt

Here we provide a detailed tutorial on CRISPR in vivo screening. Using the mouse as the model organism, we introduce a range of CRISPR tools and applications, delineate general considerations for 'transplantation-based' or 'direct in vivo' screening design, and provide details on technical execution, sequencing readouts, computational analyses and data interpretation. In vivo screens face unique pitfalls and...

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PMID: 35840661
DOI: 10.1038/s41596-022-00700-y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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