Unique dual indexing PCR reduces chimeric contamination and improves mutation detection in cell-free DNA of pregnant women.

Abstract excerpt

Allele fraction measurement is an essential component in nucleic acid analysis. The formation of chimeric amplicons during multiplex PCR amplification, however, greatly affects the allele fraction even before downstream analysis. Previous error correction strategy with unique molecular indexing (UMI) targets mainly points mutations rather than chimeras. Since the mutant allele detection in pregnant women...

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