Article
mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1.
Nature communications - 15 May 2020
Du Yushen, Hultquist Judd F, Zhou Quan, Olson Anders, Tseng Yenwen, Zhang Tian-Hao, Hong Mengying, Tang Kejun, Chen Liubo, Meng Xiangzhi, McGregor Michael J, Dai Lei, Gong Danyang, Martin-Sancho Laura, Chanda Sumit, Li Xinming, Bensenger Steve, Krogan Nevan J, Sun Ren
Abstract excerpt
A comprehensive examination of protein-protein interactions (PPIs) is fundamental for the understanding of cellular machineries. However, limitations in current methodologies often prevent the detection of PPIs with low abundance proteins. To overcome this challenge, we develop a mRNA display with library of even-distribution (md-LED) method that facilitates the detection of low abundance binders with high...
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