Article
Representative Sequencing: Unbiased Sampling of Solid Tumor Tissue.
Cell reports - 5 May 2020
Litchfield Kevin, Stanislaw Stacey, Spain Lavinia, Gallegos Lisa L, Rowan Andrew, Schnidrig Desiree, Rosenbaum Heidi, Harle Alexandre, Au Lewis, Hill Samantha M, Tippu Zayd, Thomas Jennifer, Thompson Lisa, Xu Hang, Horswell Stuart, Barhoumi Aoune, Jones Carol, Leith Katherine F, Burgess Daniel L, Watkins Thomas B K, Lim Emilia, Birkbak Nicolai J, Lamy Philippe, Nordentoft Iver, Dyrskjøt Lars, Pickering Lisa, Hazell Stephen, Jamal-Hanjani Mariam, Larkin James, Swanton Charles, Alexander Nelson R, Turajlic Samra
Abstract excerpt
Although thousands of solid tumors have been sequenced to date, a fundamental under-sampling bias is inherent in current methodologies. This is caused by a tissue sample input of fixed dimensions (e.g., 6 mm biopsy), which becomes grossly under-powered as tumor volume scales. Here, we demonstrate representative sequencing (Rep-Seq) as a new method to achieve unbiased tumor tissue sampling. Rep-Seq uses fixed...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.