A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses.

doi:10.1038/s41467-021-23779-5

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Article

A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses.

Nature communications - 8 Jun 2021

Amarilla Alberto A, Sng Julian D J, Parry Rhys, Deerain Joshua M, Potter James R, Setoh Yin Xiang, Rawle Daniel J, Le Thuy T, Modhiran Naphak, Wang Xiaohui, Peng Nias Y G, Torres Francisco J, Pyke Alyssa, Harrison Jessica J, Freney Morgan E, Liang Benjamin, McMillan Christopher L D, Cheung Stacey T M, Guevara Darwin J Da Costa, Hardy Joshua M, Bettington Mark, Muller David A, Coulibaly Fasséli, Moore Frederick, Hall Roy A, Young Paul R, Mackenzie Jason M, Hobson-Peters Jody, Suhrbier Andreas, Watterson Daniel, Khromykh Alexander A

Abstract excerpt

The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite the large size of the viral RNA genome (~30 kb), infectious full-length cDNA is readily assembled in vitro by a circular polymerase extension reaction (CPER) methodology without the need for technically demanding intermediate steps. Overlapping cDNA fragments are generated from...

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Identifiers

PMID: 34103499
DOI: 10.1038/s41467-021-23779-5
PMCID: PMC8187723

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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