Article
Rewriting the blueprint of life by synthetic genomics and genome engineering.
Genome biology - 16 Jun 2015
Annaluru Narayana, Ramalingam Sivaprakash, Chandrasegaran Srinivasan
Abstract excerpt
Advances in DNA synthesis and assembly methods over the past decade have made it possible to construct genome-size fragments from oligonucleotides. Early work focused on synthesis of small viral genomes, followed by hierarchical synthesis of wild-type bacterial genomes and subsequently on transplantation of synthesized bacterial genomes into closely related recipient strains. More recently, a synthetic designer...
Topics
- Bacteriophage T7
- Chromosomes
- Cloning, Molecular
- DNA, Bacterial
- DNA, Fungal
- DNA, Viral
- Deoxyribonucleases
- Escherichia coli
- Genetic Engineering
- Genome, Bacterial
- Genome, Fungal
- Genome, Viral
- Genomics
- Genotype
- Mycoplasma genitalium
- Phenotype
- Poliovirus
- Recombinant Proteins