Article
An E1-Catalyzed Chemoenzymatic Strategy to Isopeptide-N-Ethylated Deubiquitylase-Resistant Ubiquitin Probes.
Angewandte Chemie (International ed. in English) - 3 Aug 2020
Zheng Qingyun, Wang Tian, Chu Guo-Chao, Zuo Chong, Zhao Rui, Sui Xin, Ye Linzhi, Yu Yuanyuan, Chen Jingnan, Wu Xiangwei, Zhang Wenhao, Deng Haiteng, Shi Jing, Pan Man, Li Yi-Ming, Liu Lei
Abstract excerpt
Triazole-based deubiquitylase (DUB)-resistant ubiquitin (Ub) probes have recently emerged as effective tools for the discovery of Ub chain-specific interactors in proteomic studies, but their structural diversity is limited. A new family of DUB-resistant Ub probes is reported based on isopeptide-N-ethylated dimeric or polymeric Ub chains, which can be efficiently prepared by a one-pot, ubiquitin-activating enzyme...
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