Article
Molecular AND logic gate for multiple single-nucleotide mutations detection based on CRISPR/Cas9n system-trigged signal amplification.
Analytica chimica acta - 22 May 2020
Chang Weidan, Liu Weipeng, Shen Haoran, Chen Sisi, Liao Peizhen, Liu Yingju
Abstract excerpt
Precise detection of single-nucleotide mutations (SNMs) is extremely important in various biomedical applications, but the simultaneous detection of multiple SNMs remains a great challenge. Herein, we developed a new method based on CRISPR/Cas9 system for multiple SNMs detection. The CRISPR/Cas9 system transduces the nucleic acid into an intermediate trigger to initiate the isothermal amplification reaction and...
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