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Article

Efficient generation of zebrafish maternal-zygotic mutants through transplantation of ectopically induced and Cas9/gRNA targeted primordial germ cells.

Journal of genetics and genomics = Yi chuan xue bao - 20 Jan 2020

Zhang Fenghua, Li Xianmei, He Mudan, Ye Ding, Xiong Feng, Amin Golpour, Zhu Zuoyan, Sun Yonghua

Abstract excerpt

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology has been widely utilized for knocking out genes involved in various biological processes in zebrafish. Despite this technology is efficient for generating different mutations, one of the main drawbacks is low survival rate during embryogenesis when knocking out some embryonic lethal genes. To overcome this problem, we developed...

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Identifiers

PMID: 32094061
DOI: 10.1016/j.jgg.2019.12.004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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