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Article

Production of maternal-zygotic mutant zebrafish by germ-line replacement.

Proceedings of the National Academy of Sciences of the United States of America - 12 Nov 2002

Ciruna Brian, Weidinger Gilbert, Knaut Holger, Thisse Bernard, Thisse Christine, Raz Erez, Schier Alexander F

Abstract excerpt

We report a generally applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line. By using a morpholino oligonucleotide that blocks primordial germ cell (PGC) development, we generate embryos devoid of endogenous PGCs to serve as hosts for the transplantation of germ cells derived from homozygous mutant donors. Successful transfers are identified by the localization of...

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Identifiers

PMID: 12397179
DOI: 10.1073/pnas.222459999
PMCID: PMC137520

Genes

PGC

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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