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Article

Germline-specific dgcr8 knockout in zebrafish using a BACK approach.

Cellular and molecular life sciences : CMLS - 1 Jul 2017

Liu Yun, Zhu Zeyao, Ho Idy H T, Shi Yujian, Xie Yuxin, Li Jianzhen, Zhang Yong, Chan Matthew T V, Cheng Christopher H K

Abstract excerpt

Zebrafish is an important model to study developmental biology and human diseases. However, an effective approach to achieve spatial and temporal gene knockout in zebrafish has not been well established. In this study, we have developed a new approach, namely bacterial artificial chromosome-rescue-based knockout (BACK), to achieve conditional gene knockout in zebrafish using the Cre/loxP system. We have...

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Share to Chromosomes, Artificial, Bacterial
Share to Embryonic Development

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Knockout Techniques

Share to Gene Targeting

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Share to RNA Processing, Post-Transcriptional

Share to Transcription Activator-Like Effector Nucleases

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Source

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Identifiers

PMID: 28224202
DOI: 10.1007/s00018-017-2471-7
PMCID: PMC11107783

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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