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Article

A High-Throughput Method for Characterizing Novel Chimeric Antigen Receptors in Jurkat Cells

31 Jan 2020

Abstract excerpt

Chimeric antigen receptor (CAR) development involves extensive empirical characterization of antigen-binding domain (ABD)/CAR constructs for clinical suitability. Here, we present a cost-efficient and rapid method for evaluating CARs in human Jurkat T cells. Using a modular CAR plasmid, a highly efficient ABD cloning strategy, plasmid electroporation, short-term co-culture, and flow-cytometric detection of CD69,...

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PMID: 32083149
DOI: 10.1016/j.omtm.2020.01.012

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