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Article

Autolytic site mutant R105C of rat trypsin.

Biochemistry and molecular biology international - 1 Aug 1997

Li X F, Tang J G, Zhang L X

Abstract excerpt

The autolytic site Arg105 of rat trypsin was replaced with Cys by DNA site-directed mutagenesis method. Comparison of expression and purification of R105C trypsin along with the wild type and some other Arg105 mutants indicates that R105C trypsin could be expressed as well but with a lower expres...

Topics

Animals
Enzyme Activation
Mutagenesis, Site-Directed
Mutation
Protein Conformation
Rats
Trypsin

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 9285067
DOI: 10.1080/15216549700203441

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9285067-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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