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Article

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein.

BMC biotechnology - 20 Jan 2020

Blechert Oliver, Mei Huan, Zang Xiaohui, Zheng Hailin, Liang Guanzhao, Liu Weida

Abstract excerpt

BACKGROUND: Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80-90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools and protocols are only rudimentary. We adapt molecular genetics methods of well established fungal model organism, to knock out genes in T. rubrum. For the adaptation, crucial modifications...

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Identifiers

PMID: 31959181
DOI: 10.1186/s12896-020-0601-z
PMCID: PMC6971929

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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