Article

Trichoderma harzianum marker-free strain construction based on efficient CRISPR/Cas9 recyclable system: A helpful tool for the study of biological control agents

2023-09-01

Abstract excerpt

This article reports the first successful marker-free genetic engineering of Trichoderma harzianum, a widely studied biocontrol agent with great potential for use in sustainable agriculture. T. harzianum has been shown to control plant diseases, promote plant growth, and improve plant tolerance to abiotic stress. A specific CRISPR/Cas9 gene-editing system, based in self-replicative AMA1-plasmid, was used to genera...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 96ae26e5-feeb-5eb9-9416-8e60dc87a1f8
DOI: 10.1016/j.biocontrol.2023.105281

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Trichoderma harzianum marker-free strain construction based on efficient CRISPR/Cas9 recyclable system: A helpful tool for the study of biological control agentsDOI 10.1016/j.biocontrol.2023.105281Read

An advanced genetic toolkit for exploring the biology of the rock-inhabiting black fungus Knufia petricola.Semantic proximity Read

Efficient multiple gene knock-out in <i>Colletotrichum higginsianum</i> via CRISPR-Cas9 ribonucleoprotein and <i>URA3</i> -based marker recyclingSemantic proximity Read

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein.Semantic proximity Read

Spontaneous, Artificial, and Genome Editing-Mediated Mutations in Prunus.Semantic proximity Read

Colony morphology mutants of Trichoderma harzianum with increased beta-1,4-N-acetyl-glucosaminidase production.Semantic proximity Read

A comparison of the phenotypic and genetic stability of recombinant Trichoderma spp. generated by protoplast- and Agrobacterium-mediated transformation.Semantic proximity Read

Protocol of Phytophthora capsici Transformation Using the CRISPR-Cas9 System.Semantic proximity Read

Harnessing the potential of mutation breeding, CRISPR genome editing, and beyond for sustainable agriculture.Semantic proximity Read

A CRISPR-Cas9 System for Genome Editing of Fusarium proliferatum.Semantic proximity Read

Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System.Semantic proximity Read

Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia.Semantic proximity Read

Non-homologous end-joining-deficient filamentous fungal strains mitigate the impact of off-target mutations during the application of CRISPR/Cas9.Semantic proximity Read