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Introducing multiple-gene mutations in Pleurotus ostreatus using a polycistronic tRNA and CRISPR guide RNA strategy.

FEMS microbiology letters - 9 Nov 2022

Xu Haibo, Nakazawa Takehito, Zhang Yufan, Oh Minji, Bao Dapeng, Kawauchi Moriyuki, Sakamoto Masahiro, Honda Yoichi

Abstract excerpt

The white-rot fungus Pleurotus ostreatus is an agaricomycete that is frequently used in molecular genetics studies as many useful tools are applicable to the fungus. In particular, efficient gene targeting using homologous recombination and CRISPR/Cas9 enables the introduction of a mutation in the gene of interest for functional analysis. Multiple genes encoding various lignocellulose-degrading enzymes are...

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PMID: 36302144
DOI: 10.1093/femsle/fnac102

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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