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Article

Measuring Microbial Mutation Rates with the Fluctuation Assay.

Journal of visualized experiments : JoVE - 28 Nov 2019

Krašovec Rok, Richards Huw, Gomez Guillaume, Gifford Danna R, Mazoyer Adrien, Knight Christopher G

Abstract excerpt

Fluctuation assays are widely used for estimating mutation rates in microbes growing in liquid environments. Many cultures are each inoculated with a few thousand cells, each sensitive to a selective marker that can be assayed phenotypically. These parallel cultures grow for many generations in the absence of the phenotypic marker. A subset of cultures is used to estimate the total number of cells at risk of...

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PMID: 31840662
DOI: 10.3791/60406

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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