Article
Determination of Spontaneous Mutation Rate by Fluctuation Analysis Using 5-Fluoroorotic Acid (5-FOA).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026
Milisavljevic Mira, Kojic Milorad
Abstract excerpt
Spontaneous mutations serve as critical indicators of genome stability. Regardless of the mechanism, the rate of mutation in a certain genetic background can often provide insight into the underlying biological processes that impact the accuracy of DNA replication or the efficacy of DNA repair. A common method for estimating mutation rates is the Luria-Delbrück fluctuation assay. Here, a protocol is presented for...
Read the complete abstract on PubMedTopics
- Orotic Acid
- Mutation Rate
- Mutation
- DNA Replication