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Multiplex analysis of antigen-specific memory B cells in humans using reversed B-cell FluoroSpot

3 Dec 2019

Abstract excerpt

Analysis of B-cell specificities at the single cell level provides important information on how the B-cell compartment responds when challenged by infection or vaccination. We recently developed a reversed B-cell FluoroSpot assay and showed that it could be used to detect B cells specific for different antigens simultaneously in a mouse model. The aim of this study was to further develop the method to detect and...

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PMID: 31809709
DOI: 10.1016/j.jim.2019.112715

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