Article

Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity

2023-03-10

Abstract excerpt

Bacterial capsular polysaccharides are important vaccine immunogens. However, the study of polysaccharide-specific immune responses has been hindered by technical restrictions. Here, we developed and validated a high-throughput method to analyse antigen-specific B cells using combinatorial staining with fluorescently-labelled capsular polysaccharide multimers. Concurrent staining of 25+ cellular markers further en...

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Literature Corpus work: 2486a187-8909-5fe8-9f85-de7e0909dc40
DOI: 10.1101/2023.03.07.531507

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Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunityDOI 10.1101/2023.03.07.531507Read

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