Article

A whole blood intracellular cytokine assay optimised for field site studies demonstrates polyfunctionality of CD4+ T cells in acute scrub typhus

2022-10-28

Abstract excerpt

<h4>Background</h4> Assessment of cellular immune responses by combining intracellular cytokine staining and immunophenotyping using flow cytometry enables the simultaneous measurement of T cell phenotype and effector function in response to pathogens and vaccines. The use of whole blood samples rather than peripheral blood mononuclear cells avoids both the need for immediate processing and loss of functional ant...

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Literature Corpus work: e530c3cb-062d-57a6-89e5-8fcf79a42190
DOI: 10.1101/2022.10.28.513456

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