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Article

Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT.

Scientific reports - 27 Nov 2017

Shen Chuanlai, Xu Tao, Wu You, Li Xiaoe, Xia Lingzhi, Wang Wei, Shahzad Khawar Ali, Zhang Lei, Wan Xin, Qiu Jie

Abstract excerpt

Conventional peptide-major histocompatibility complex (pMHC) multimer staining, intracellular cytokine staining, and enzyme-linked immunospot (ELISPOT) assay cannot concurrently determine the frequency and reactivity of antigen-specific T cells (AST) in a single assay. In this report, pMHC multimer, magnetic-activated cell sorting (MACS), and ELISPOT techniques have been integrated into a micro well by coupling...

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Identifiers

PMID: 29180767
DOI: 10.1038/s41598-017-16549-1
PMCID: PMC5703716

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