HLA-B*27 typing by PCR-restriction fragment length polymorphism.

doi:10.1111/j.1399-0039.1997.tb02754.x

Back to search

Article

HLA-B*27 typing by PCR-restriction fragment length polymorphism.

Tissue antigens - 1 Mar 1997

Nieto A, Fraile A, Vinasco J, Martín J

Abstract excerpt

In this study we describe the use of PCR-RFLP for genotyping HLA-B*27. A 557 bp fragment from HLA-B locus is amplified and subjected to digestion with StyI. The presence of B*27 is detected on electrophoresis by the appearance of 431 + 126 bp pattern. The same pattern could be obtained only for t...

Topics

Cell Line
Genotype
HLA-B27 Antigen
Histocompatibility Testing
Humans
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.