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Article

Rational design of a thermophilic β-mannanase fromBacillus subtilis TJ-102 to improve its thermostability.

Enzyme and microbial technology - 1 Nov 2018

Wang Xiang-Chao, You Sheng-Ping, Zhang Jia-Xing, Dai Ye-Mei, Zhang Cheng-Yu, Qi Wei, Dou Tong-Yi, Su Rong-Xin, He Zhi-Min

Abstract excerpt

A rational design method to improve β-mannanase (ManTJ102) thermostability was developed successfully in this study. The flexible area of residues 330-340 in ManTJ102 was firstly selected from analysis of molecular dynamics simulation and then the critical amino acid residue (Ala336Pro) with the lowest mutation energy was determined by virtual mutation, whose mutant was named as Mutant336. Afterward, the dynamics...

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PMID: 30143199
DOI: 10.1016/j.enzmictec.2018.07.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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