A multi-factors rational design strategy for enhancing the thermostability of Escherichia coli AppA phytase.

Abstract excerpt

Despite recent advances in our understanding of the importance of protein surface properties for protein thermostability,there are seldom studies on multi-factors rational design strategy, so a more scientific, simple and effective rational strategy is urgent for protein engineering. Here, we first attempted to use a three-factors rational design strategy combining three common structural features, protein...

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