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Article

Improved sensitivity for solid-support invasive cleavage reactions with flow cytometry analysis.

BioTechniques - 1 Jan 2003

Stevens P Wilkins, Rao K V N, Hall J G, Lyamichev V, Neri B P, Kelso D M

Abstract excerpt

A new configuration of the solid-support invasive cleavage reaction provides a small reaction-volume format for high-sensitivity discrimination of nucleic acid targets with single nucleotide differences. With target concentrations as low as 2 amol/assay, the solid-support invasive cleavage reaction clearly distinguishes single base mutations. Two oligonucleotides tethered to the solid support hybridize to the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 12545560
DOI: 10.2144/03341dd09

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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