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Transcriptomic analysis of IgG4 Fc‐fusion protein degradation in a panel of clonally‐derived CHO cell lines using RNASeq

26 Feb 2019

Abstract excerpt

In this study, we report an investigation of a panel of clonally-derived Chinese hamster ovary (CHO) cell lines exhibiting variability in the proportion of full-length IgG4 Fc-fusion protein produced. The recombinant protein was found to be degraded during cell culture into four shorter "clipped" species (three of the four cleavage sites occurred at arginine residues) and preliminary analyses suggested that a...

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PMID: 30802296
DOI: 10.1002/bit.26958

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