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Article

Identifying low-level sequence variants via next generation sequencing to aid stable CHO cell line screening.

Biotechnology progress - 1 Jan 2000

Zhang Sheng, Bartkowiak Lisa, Nabiswa Bernard, Mishra Pratibha, Fann John, Ouellette David, Correia Ivan, Regier Dean, Liu Junjian

Abstract excerpt

Developing stable Chinese hamster ovary (CHO) cell lines for biotherapeutics is an irreversible process and therefore, key quality attributes, such as sequence variants, must be closely monitored during cell line development (CLD) to avoid delay in the developmental timeline, and more importantly, to assure product safety and efficacy. Sequence variants, defined as unintended amino acid substitution in...

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PMID: 26033952
DOI: 10.1002/btpr.2119

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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