Article
Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato.
Scientific reports - 13 Sept 2018
Kusano Hiroaki, Ohnuma Mariko, Mutsuro-Aoki Hiromi, Asahi Takahiro, Ichinosawa Dai, Onodera Hitomi, Asano Kenji, Noda Takahiro, Horie Takaaki, Fukumoto Kou, Kihira Miho, Teramura Hiroshi, Yazaki Kazufumi, Umemoto Naoyuki, Muranaka Toshiya, Shimada Hiroaki
Abstract excerpt
CRISPR/Cas9 is a programmable nuclease composed of the Cas9 protein and a guide RNA (gRNA) molecule. To create a mutant potato, a powerful genome-editing system was required because potato has a tetraploid genome. The translational enhancer dMac3, consisting of a portion of the OsMac3 mRNA 5'-untranslated region, greatly enhanced the production of the protein encoded in the downstream ORF. To enrich the amount of...
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