Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts.

Plant cell reports - 1 Jan 2017

Andersson Mariette, Turesson Helle, Nicolia Alessandro, Fält Ann-Sofie, Samuelsson Mathias, Hofvander Per

Abstract excerpt

KEY MESSAGE: Altered starch quality with full knockout of GBSS gene function in potato was achieved using CRISPR-Cas9 technology, through transient transfection and regeneration from isolated protoplasts. Site-directed mutagenesis (SDM) has shown great progress in introducing precisely targeted mutations. Engineered CRISPR-Cas9 has received increased focus compared to other SDM techniques, since the method is...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Cas Systems
Share to Genotyping Techniques

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mutagenesis

Share to Protoplasts

Share to Regeneration

Share to Solanum tuberosum

Share to Starch

Share to Tetraploidy

Source

Open in PubMed Open full text

Identifiers

PMID: 27699473
DOI: 10.1007/s00299-016-2062-3
PMCID: PMC5206254

Genes

PAM

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts.PMID 27699473Read ↗

Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System.Semantic proximity Read ↗

Facilitating gene editing in potato: a Single-Nucleotide Polymorphism (SNP) map of the Solanum tuberosum L. cv. Desiree genome.Semantic proximity Read ↗

Prime editing is achievable in the tetraploid potato, but needs improvementSemantic proximity Read ↗

Protoplast-Based Method for Genome Editing in Tetraploid Potato.Semantic proximity Read ↗

Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato.Semantic proximity Read ↗

Colour change in potato (Solanum tuberosum L.) tubers by disruption of the anthocyanin pathway via ribonucleoprotein complex delivery of the CRISPR/Cas9 systemSemantic proximity Read ↗

Functional inhibition of the StERF3 gene by dual targeting through CRISPR/Cas9 enhances resistance to the late blight disease in Solanum Tuberosum L.Semantic proximity Read ↗

Glycoalkaloid-Free Starch Potatoes Generated by CRISPR/Cas9-Mediated Mutations of Genes in the Glycoalkaloid Biosynthesis Pathway Enable More Sustainable Uses of By-Products From Starch Production.Semantic proximity Read ↗

Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato.Semantic proximity Read ↗

Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation.Semantic proximity Read ↗

Recent Advances in Molecular Improvement for Potato Tuber Traits.Semantic proximity Read ↗

Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.