Article
Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange.
Plant cell reports - 1 Dec 2021
Tang Xiaomei, Chen Shulin, Yu Huiwen, Zheng Xiongjie, Zhang Fei, Deng Xiuxin, Xu Qiang
Abstract excerpt
KEY MESSAGE: Here, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by...
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