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Article

Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato.

Scientific reports - 22 Feb 2021

Zhao Xue, Jayarathna Shishanthi, Turesson Helle, Fält Ann-Sofie, Nestor Gustav, González Matías N, Olsson Niklas, Beganovic Mirela, Hofvander Per, Andersson Roger, Andersson Mariette

Abstract excerpt

DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency up to 72% was achieved. The large variation of mutations was grouped as follows: Group 1 lines with all alleles of Sbe1...

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Identifiers

PMID: 33619312
DOI: 10.1038/s41598-021-83462-z
PMCID: PMC7900246

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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