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Article

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis.

Plant biotechnology journal - 1 Feb 2016

Mao Yanfei, Zhang Zhengjing, Feng Zhengyan, Wei Pengliang, Zhang Hui, Botella José Ramón, Zhu Jian-Kang

Abstract excerpt

The Streptococcus-derived CRISPR/Cas9 system is being widely used to perform targeted gene modifications in plants. This customized endonuclease system has two components, the single-guide RNA (sgRNA) for target DNA recognition and the CRISPR-associated protein 9 (Cas9) for DNA cleavage. Ubiquitously expressed CRISPR/Cas9 systems (UC) generate targeted gene modifications with high efficiency but only those...

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PMID: 26360626
DOI: 10.1111/pbi.12468
PMCID: PMC5515382

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GSC

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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