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Target-specific mutations efficiency at multiple loci of CRISPR/Cas9 system using one sgRNA in soybean.

Transgenic research - 1 Feb 2021

Zhang Ling, Wang Yingzhe, Li Tong, Qiu Hongmei, Xia Zhengjun, Dong Yingshan

Abstract excerpt

Soybean has a palaeopolyploid genome with nearly 75% of the genes present in multiple copies. Although the CRISPR/Cas9 system has been employed in soybean to generate site-directed mutagenesis, a systematical assessment of mutation efficiency of the CRISPR/Cas9 system for the multiple-copy genes is still urgently needed. Here, we successfully optimize one sgRNA CRISPR/Cas9 system in soybean by testing the...

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CRISPR-Cas Systems
Fatty Acid Desaturases
Gene Editing
Genome, Plant
Glycine max

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oxidoreductases

Plants, Genetically Modified

RNA, Guide, CRISPR-Cas Systems

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PMID: 33387102
DOI: 10.1007/s11248-020-00228-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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