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Site-Directed Mutagenesis to Mutate Multiple Residues in a Single Reaction.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2024

Usman Saima, Bushaala Antesar, Teh Muy Teck, Waseem Ahmad

Abstract excerpt

Site-directed mutagenesis (SDM) is a technique that allows mutation of specific nucleotide(s) in a codon to study its functional implications in a protein. Commercial kits are available, which require high-performance liquid chromatography purified oligos for this purpose. These kits are expensive, and they are not very efficient, so one has to sequence several clones to get a desired one. We present here a...

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PMID: 38180689
DOI: 10.1007/7651_2023_511

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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