Article

Benchmarking of proximity-dependent biotinylation enzymes across cellular compartments and time windows

2025-08-30

Abstract excerpt

Proximity-dependent biotinylation has become a powerful approach for mapping protein interactions and subcellular organization in living cells. Although a growing number of engineered biotin ligases have been introduced, their performance has not been systematically evaluated across diverse cellular contexts. Here, we benchmark ten proximity ligases spanning three bacterial lineages using standardized proteomic wo...

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Literature Corpus work: fcf135c5-e5bb-5f10-a6f3-37f592ffb02c
DOI: 10.1101/2025.08.29.668156

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