Article
A Comparison Between Full-COLD PCR/HRM and PCR Sequencing for Detection of Mutations in Exon 9 of PIK3CA in Breast Cancer Patients.
Applied biochemistry and biotechnology - 1 Mar 2019
Ghalamkari Safoura, Khosravian Farinaz, Mianesaz Hamidreza, Kazemi Mohammad, Behjati Mohaddeseh, Hakimian Sayyed Mohammadreza, Salehi Mansoor
Abstract excerpt
One of the most common somatic mutations in breast cancer is found in PIK3CA with a prevalence rate of 18-45%. Different variants of this gene are considered as resistance markers for treatment with HER2-targeted medicines. Conventional molecular methods such as Sanger sequencing are not able to detect mutations with low abundance in a mixture of wild-type DNA, especially in the early stages of cancer...
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