Article
A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes.
Nucleic acids research - 22 Sept 2023
Yang Ya-Jun, Fu Hang, Li Xiao-Lu, Yang Hong-Yu, Zhou Er-Chi, Xie Cheng-Yu, Wu Shu-Wen, He Fan, Zhang Yan, Zhang Xing-Hua
Abstract excerpt
The detection of nucleic acid sequences in parallel with the discrimination of single nucleotide variations (SNVs) is critical for research and clinical applications. A few limitations make the detection technically challenging, such as too small variation in probe-hybridization energy caused by SNVs, the non-specific amplification of false nucleic acid fragments and the few options of dyes limited by spectral...
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