Article
Shared nucleotide flanks confer transcriptional competency to bZip core motifs.
Nucleic acids research - 19 Sept 2018
Cohen Daniel M, Lim Hee-Woong, Won Kyoung-Jae, Steger David J
Abstract excerpt
Sequence-specific DNA binding recruits transcription factors (TFs) to the genome to regulate gene expression. Here, we perform high resolution mapping of CEBP proteins to determine how sequence dictates genomic occupancy. We demonstrate a fundamental difference between the sequence repertoire utilized by CEBPs in vivo versus the palindromic sequence preference reported by classical in vitro models, by identifying...
Topics
- Animals
- Base Sequence
- Basic-Leucine Zipper Transcription Factors
- Binding Sites
- CCAAT-Enhancer-Binding Proteins
- Chromatin Immunoprecipitation
- Dimerization
- High-Throughput Nucleotide Sequencing
- Humans
- Mice
- Nucleotide Motifs
- Polymorphism, Single Nucleotide
- Protein Binding
- Specific Pathogen-Free Organisms